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dh5α  (New England Biolabs)


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    New England Biolabs dh5α
    Dh5α, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3654 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5+alpha+competent+e+coli/NEB+5-alpha+Competent+E%2E+coli/pmc12796543-239-6-7
    Average 99 stars, based on 3654 article reviews
    dh5α - by Bioz Stars, 2026-09
    99/100 stars

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    Cloning:

    Article Title: Chromatin dynamics of the Klf4 locus in mouse pluripotent cells.
    Article Snippet: .. All cloning reactions were carried out with 25 μl of NEB 5-alpha Competent E. coli (New England Biolabs, Catalog no: C2987H) using heat-shock at 42°C. .. Transformants were picked and plasmid DNA was purified with the QIAprep Spin Miniprep Kit (QIAGEN, Catalog no: 27106) and/or the NucleoBond Xtra Midi kit (MACHEREY-NAGEL, Catalog no: 740410.50).

    Purification:

    Article Title: Precision Fermentation of Recombinant Myofibrillar Proteins for Future Foods
    Article Snippet: PCR reactions were carried out using NEB Q5 High-Fidelity DNA Polymerase (NEB #M0491) and dNTPs (NEB #N0447). .. Plasmids were expanded using 5-alpha competent E. coli (NEB #C2987) in LB Broth (Sigma Aldrich, St. Luois, MO, #3522) with kanamycin 50μg/mL (Sigma Aldrich #K4000) as antibiotic, and purified using Macherey-Nagel (Düren, Germany) NucleoSpin Plamid Mini kit (#740588). .. Plasmid insertions were verified using Azenta (Burlington, MA) Genewiz Sanger sequencing service using T7 promoter and terminator as forward and reverse primers, respectively.

    Article Title: Mutations that positively affect Bandavirus glycoprotein function on VSV vectored vaccines
    Article Snippet: PCR products were purified from agarose gels using Monarch DNA Gel Extraction Kit (New England Biolabs, T1020) using manufacturer’s protocol and ligated using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, E2621). .. All plasmids were grown in NEB 5-alpha Competent E. coli (New England Biolabs, C2987) and purified using ZymoPURE Plasmid Miniprep Kit (Zymo research, D4210) or GenElute HP Plasmid Maxiprep Kit (Sigma-Aldrich, NA0310-1KT). .. All plasmids were submitted for Nanopore sequencing prior to use (Eurofins Genomics).

    Plasmid Preparation:

    Article Title: Mutations that positively affect Bandavirus glycoprotein function on VSV vectored vaccines
    Article Snippet: PCR products were purified from agarose gels using Monarch DNA Gel Extraction Kit (New England Biolabs, T1020) using manufacturer’s protocol and ligated using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, E2621). .. All plasmids were grown in NEB 5-alpha Competent E. coli (New England Biolabs, C2987) and purified using ZymoPURE Plasmid Miniprep Kit (Zymo research, D4210) or GenElute HP Plasmid Maxiprep Kit (Sigma-Aldrich, NA0310-1KT). .. All plasmids were submitted for Nanopore sequencing prior to use (Eurofins Genomics).

    Article Title: Sex distorter male drive for resistance-resilient population control of the human malaria vector Anopheles gambiae.
    Article Snippet: Constructs cloned using Golden Gate assembly (T4 DNA ligase and buffer New England Biolabs, UK; FastDigest Eco31I Thermo ScientificTM, UK) were transformed in InvitrogenTM One ShotTM TOP10 Chemically Competent E. coli (Fisher Scientific, UK). .. NEB 5-alpha Competent E. coli #C2987 (New England Biolabs, UK) were used to transform plasmid assembled through Gibson assembly (Gibson Assembly Master Mix NEB #M5510, New England Biolabs, UK). ..

    Transformation Assay:

    Article Title: CHARIOT-AAV: Conjugation of diverse vectors to adeno-associated viruses for delivery of large genes
    Article Snippet: N1-Methylpseudouridine (N1mU)-modified mRNAs encoding Cre recombinase and enhanced green fluorescent protein (eGFP) were purchased from TriLink Biotechnologies (#L-8111) and GenScript, respectively (#RP-A00009). .. NEB 5-alpha Competent E. coli (High Efficiency; NEB C2987H) was used in transformation for all genetic constructs. .. Miniprep was performed using NucleoSpin Plasmid EasyPure (Takara Bio #740727.50).

    Article Title: Decreasing peptide deformylase activity is a beneficial strategy for increasing formaldehyde resistance in Methylobacterium extorquens
    Article Snippet: Plasmid backbones (pCH07 and pET28a) were linearized by restriction digest and insertion fragments were inserted with an NEBuilder ® DNA Hifi Assembly reaction at 50 °C for 1 hr. .. Assemblies were transformed into NEB ® 5-alpha Competent E. coli (C2987). ..

    Article Title: Designing genome editing experiments with EditABLE.
    Article Snippet: We phosphorylated and annealed the guide RNA oligonucleotides using T4 polynucleotide kinase (NEB #M0201S) at 37oC for 30 minutes, and we ligated the annealed oligonucleotides with the digested mCherry-U6-empty plasmid using T4 DNA ligase (NEB #M0202S) for 2 hours at 25oC. .. Lastly, we transformed the ligation product into 5-alpha competent E. coli (NEB, # C2987I) based on the manufacturer’s instructions and sequence verified individual colonies resulting from the transformation. .. We isolated plasmid DNA from verified colonies using the ZymoPURETM II Plasmid Purification Maxiprep Kit (Zymo Research #D4202, Irvine, CA).

    Construct:

    Article Title: CHARIOT-AAV: Conjugation of diverse vectors to adeno-associated viruses for delivery of large genes
    Article Snippet: N1-Methylpseudouridine (N1mU)-modified mRNAs encoding Cre recombinase and enhanced green fluorescent protein (eGFP) were purchased from TriLink Biotechnologies (#L-8111) and GenScript, respectively (#RP-A00009). .. NEB 5-alpha Competent E. coli (High Efficiency; NEB C2987H) was used in transformation for all genetic constructs. .. Miniprep was performed using NucleoSpin Plasmid EasyPure (Takara Bio #740727.50).

    Ligation:

    Article Title: Designing genome editing experiments with EditABLE.
    Article Snippet: We phosphorylated and annealed the guide RNA oligonucleotides using T4 polynucleotide kinase (NEB #M0201S) at 37oC for 30 minutes, and we ligated the annealed oligonucleotides with the digested mCherry-U6-empty plasmid using T4 DNA ligase (NEB #M0202S) for 2 hours at 25oC. .. Lastly, we transformed the ligation product into 5-alpha competent E. coli (NEB, # C2987I) based on the manufacturer’s instructions and sequence verified individual colonies resulting from the transformation. .. We isolated plasmid DNA from verified colonies using the ZymoPURETM II Plasmid Purification Maxiprep Kit (Zymo Research #D4202, Irvine, CA).

    Sequencing:

    Article Title: Designing genome editing experiments with EditABLE.
    Article Snippet: We phosphorylated and annealed the guide RNA oligonucleotides using T4 polynucleotide kinase (NEB #M0201S) at 37oC for 30 minutes, and we ligated the annealed oligonucleotides with the digested mCherry-U6-empty plasmid using T4 DNA ligase (NEB #M0202S) for 2 hours at 25oC. .. Lastly, we transformed the ligation product into 5-alpha competent E. coli (NEB, # C2987I) based on the manufacturer’s instructions and sequence verified individual colonies resulting from the transformation. .. We isolated plasmid DNA from verified colonies using the ZymoPURETM II Plasmid Purification Maxiprep Kit (Zymo Research #D4202, Irvine, CA).



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    Image Search Results


    UBA7 variants from individuals with neurodevelopmental disorders alter protein structure (A) Pedigrees of the families studied in this article indicate the affected individuals 1, 2, and 3. A legend is provided for the symbols used. (B) Diagram of UBA7 protein domains and their functions. The location of the identified variants in the structure is indicated. IAD: inactive adenylation domain, AAD: active adenylation domain, FCCH: first catalytic cysteine half-domain, SCCH: second catalytic cysteine half-domain, UFD: ubiquitin-fold domain. (C) Alpha-fold models of UBA7 protein resulting from UBA7 wild type, or p.Trp311∗ and p.Lys709Serfs∗45 variants. The Red helix represents the added residues resulting from the p.Lys709Serfs∗45 frameshift variant. (D) Structure of wild-type UBA7 in complex with UBE2L6 and ISG15 (left; EMB-16891; pdb: 8OIF ). The top-view (middle) and zoomed areas (right) highlight the adenylation pocket and location of the p.Val548Leu mutation shown in red.

    Journal: iScience

    Article Title: ISGylation is disrupted by UBA7 gene variants identified in individuals with neurodevelopmental disorder phenotypes

    doi: 10.1016/j.isci.2026.115454

    Figure Lengend Snippet: UBA7 variants from individuals with neurodevelopmental disorders alter protein structure (A) Pedigrees of the families studied in this article indicate the affected individuals 1, 2, and 3. A legend is provided for the symbols used. (B) Diagram of UBA7 protein domains and their functions. The location of the identified variants in the structure is indicated. IAD: inactive adenylation domain, AAD: active adenylation domain, FCCH: first catalytic cysteine half-domain, SCCH: second catalytic cysteine half-domain, UFD: ubiquitin-fold domain. (C) Alpha-fold models of UBA7 protein resulting from UBA7 wild type, or p.Trp311∗ and p.Lys709Serfs∗45 variants. The Red helix represents the added residues resulting from the p.Lys709Serfs∗45 frameshift variant. (D) Structure of wild-type UBA7 in complex with UBE2L6 and ISG15 (left; EMB-16891; pdb: 8OIF ). The top-view (middle) and zoomed areas (right) highlight the adenylation pocket and location of the p.Val548Leu mutation shown in red.

    Article Snippet: NEB 5-alpha Competent E. coli (DH5α) , New England Biolabs , Cat# C2987.

    Techniques: Ubiquitin Proteomics, Variant Assay, Mutagenesis